how to set micropiptte volume with decimal separators( like) how can I set 109.5 in p200?
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And how can I set 150.1 in p200 ,,871.3 in p1000,,840.5 in p1000 ??
molecular-biology
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And how can I set 150.1 in p200 ,,871.3 in p1000,,840.5 in p1000 ??
molecular-biology
asked 9 hours ago
SaraSara
161
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Sara is a new contributor to this site. Take care in asking for clarification, commenting, and answering.
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add a comment |
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And how can I set 150.1 in p200 ,,871.3 in p1000,,840.5 in p1000 ??
molecular-biology
asked 9 hours ago
SaraSara
161
New contributor
Sara is a new contributor to this site. Take care in asking for clarification, commenting, and answering.
Check out our Code of Conduct.
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And how can I set 150.1 in p200 ,,871.3 in p1000,,840.5 in p1000 ??
molecular-biology
molecular-biology
asked 9 hours ago
SaraSara
161
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Sara is a new contributor to this site. Take care in asking for clarification, commenting, and answering.
Check out our Code of Conduct.
asked 9 hours ago
SaraSara
161
New contributor
Sara is a new contributor to this site. Take care in asking for clarification, commenting, and answering.
Check out our Code of Conduct.
asked 9 hours ago
SaraSara
161
New contributor
Sara is a new contributor to this site. Take care in asking for clarification, commenting, and answering.
Check out our Code of Conduct.
asked 9 hours ago
SaraSara
161
asked 9 hours ago
SaraSara
161
161
New contributor
Sara is a new contributor to this site. Take care in asking for clarification, commenting, and answering.
Check out our Code of Conduct.
New contributor
Sara is a new contributor to this site. Take care in asking for clarification, commenting, and answering.
Check out our Code of Conduct.
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Check out our Code of Conduct.
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add a comment |
2 Answers
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active
oldest
votes
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It doesn't make sense to set volumes of decimals of microliters with a P200, since usually the accuracy of these pipettes is in the order of a few microliters. So even if you set your pipette at 190 uL, you may still dispense a volume ranging from 187 to 193 uL. For a P1000, due to higher volumes the inaccuracy is of course higher, usually +/- 10 uL or more. Some brands are more accurate than others, like Eppendorf or Finnpipette. I suggest you check the specs of your pipettes and see how much accuracy you can achieve.
answered 9 hours ago
betelgeusebetelgeuse
862
$endgroup$
1
$begingroup$
I think a fair take-home message here is you shouldn't, and with many brands you often can't. Rainin and Eppendorf P1000's measure to four places from 0100 to 1000, for example. The best bet is to use two different pipets, and use them in that fashion throughout the process to maintain consistency.
$endgroup$
– CKM
6 hours ago
add a comment |
$begingroup$
For p200 the smallest unit is the tenth of a ul (0.1) but the intervals go by 0.2 so to set 109.5 (assuming your pipette can be adjusted between intervals) you need to set the pipette so the pointer is equidistant from the 109.4 and 109.6 mark. For the p1000 the smallest unit is 1 ul so you cannot set it to 871.3.
answered 6 hours ago
CellCell
362116
$endgroup$
$begingroup$
Some brands of pipeters "click" at each allowed increment, so you won't be able to go between them.
$endgroup$
– swbarnes2
4 hours ago
$begingroup$
Interesting. Thanks for sharing I didn't know that.
$endgroup$
– Cell
2 hours ago
add a comment |
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2 Answers
2
active
oldest
votes
2 Answers
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active
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votes
$begingroup$
It doesn't make sense to set volumes of decimals of microliters with a P200, since usually the accuracy of these pipettes is in the order of a few microliters. So even if you set your pipette at 190 uL, you may still dispense a volume ranging from 187 to 193 uL. For a P1000, due to higher volumes the inaccuracy is of course higher, usually +/- 10 uL or more. Some brands are more accurate than others, like Eppendorf or Finnpipette. I suggest you check the specs of your pipettes and see how much accuracy you can achieve.
answered 9 hours ago
betelgeusebetelgeuse
862
$endgroup$
1
$begingroup$
I think a fair take-home message here is you shouldn't, and with many brands you often can't. Rainin and Eppendorf P1000's measure to four places from 0100 to 1000, for example. The best bet is to use two different pipets, and use them in that fashion throughout the process to maintain consistency.
$endgroup$
– CKM
6 hours ago
add a comment |
$begingroup$
It doesn't make sense to set volumes of decimals of microliters with a P200, since usually the accuracy of these pipettes is in the order of a few microliters. So even if you set your pipette at 190 uL, you may still dispense a volume ranging from 187 to 193 uL. For a P1000, due to higher volumes the inaccuracy is of course higher, usually +/- 10 uL or more. Some brands are more accurate than others, like Eppendorf or Finnpipette. I suggest you check the specs of your pipettes and see how much accuracy you can achieve.
answered 9 hours ago
betelgeusebetelgeuse
862
$endgroup$
1
$begingroup$
I think a fair take-home message here is you shouldn't, and with many brands you often can't. Rainin and Eppendorf P1000's measure to four places from 0100 to 1000, for example. The best bet is to use two different pipets, and use them in that fashion throughout the process to maintain consistency.
$endgroup$
– CKM
6 hours ago
add a comment |
$begingroup$
It doesn't make sense to set volumes of decimals of microliters with a P200, since usually the accuracy of these pipettes is in the order of a few microliters. So even if you set your pipette at 190 uL, you may still dispense a volume ranging from 187 to 193 uL. For a P1000, due to higher volumes the inaccuracy is of course higher, usually +/- 10 uL or more. Some brands are more accurate than others, like Eppendorf or Finnpipette. I suggest you check the specs of your pipettes and see how much accuracy you can achieve.
answered 9 hours ago
betelgeusebetelgeuse
862
$endgroup$
It doesn't make sense to set volumes of decimals of microliters with a P200, since usually the accuracy of these pipettes is in the order of a few microliters. So even if you set your pipette at 190 uL, you may still dispense a volume ranging from 187 to 193 uL. For a P1000, due to higher volumes the inaccuracy is of course higher, usually +/- 10 uL or more. Some brands are more accurate than others, like Eppendorf or Finnpipette. I suggest you check the specs of your pipettes and see how much accuracy you can achieve.
answered 9 hours ago
betelgeusebetelgeuse
862
answered 9 hours ago
betelgeusebetelgeuse
862
answered 9 hours ago
betelgeusebetelgeuse
862
answered 9 hours ago
betelgeusebetelgeuse
862
862
1
$begingroup$
I think a fair take-home message here is you shouldn't, and with many brands you often can't. Rainin and Eppendorf P1000's measure to four places from 0100 to 1000, for example. The best bet is to use two different pipets, and use them in that fashion throughout the process to maintain consistency.
$endgroup$
– CKM
6 hours ago
add a comment |
1
$begingroup$
I think a fair take-home message here is you shouldn't, and with many brands you often can't. Rainin and Eppendorf P1000's measure to four places from 0100 to 1000, for example. The best bet is to use two different pipets, and use them in that fashion throughout the process to maintain consistency.
$endgroup$
– CKM
6 hours ago
1
1
$begingroup$
I think a fair take-home message here is you shouldn't, and with many brands you often can't. Rainin and Eppendorf P1000's measure to four places from 0100 to 1000, for example. The best bet is to use two different pipets, and use them in that fashion throughout the process to maintain consistency.
$endgroup$
– CKM
6 hours ago
$begingroup$
I think a fair take-home message here is you shouldn't, and with many brands you often can't. Rainin and Eppendorf P1000's measure to four places from 0100 to 1000, for example. The best bet is to use two different pipets, and use them in that fashion throughout the process to maintain consistency.
$endgroup$
– CKM
6 hours ago
add a comment |
$begingroup$
For p200 the smallest unit is the tenth of a ul (0.1) but the intervals go by 0.2 so to set 109.5 (assuming your pipette can be adjusted between intervals) you need to set the pipette so the pointer is equidistant from the 109.4 and 109.6 mark. For the p1000 the smallest unit is 1 ul so you cannot set it to 871.3.
answered 6 hours ago
CellCell
362116
$endgroup$
$begingroup$
Some brands of pipeters "click" at each allowed increment, so you won't be able to go between them.
$endgroup$
– swbarnes2
4 hours ago
$begingroup$
Interesting. Thanks for sharing I didn't know that.
$endgroup$
– Cell
2 hours ago
add a comment |
$begingroup$
For p200 the smallest unit is the tenth of a ul (0.1) but the intervals go by 0.2 so to set 109.5 (assuming your pipette can be adjusted between intervals) you need to set the pipette so the pointer is equidistant from the 109.4 and 109.6 mark. For the p1000 the smallest unit is 1 ul so you cannot set it to 871.3.
answered 6 hours ago
CellCell
362116
$endgroup$
$begingroup$
Some brands of pipeters "click" at each allowed increment, so you won't be able to go between them.
$endgroup$
– swbarnes2
4 hours ago
$begingroup$
Interesting. Thanks for sharing I didn't know that.
$endgroup$
– Cell
2 hours ago
add a comment |
$begingroup$
For p200 the smallest unit is the tenth of a ul (0.1) but the intervals go by 0.2 so to set 109.5 (assuming your pipette can be adjusted between intervals) you need to set the pipette so the pointer is equidistant from the 109.4 and 109.6 mark. For the p1000 the smallest unit is 1 ul so you cannot set it to 871.3.
answered 6 hours ago
CellCell
362116
$endgroup$
For p200 the smallest unit is the tenth of a ul (0.1) but the intervals go by 0.2 so to set 109.5 (assuming your pipette can be adjusted between intervals) you need to set the pipette so the pointer is equidistant from the 109.4 and 109.6 mark. For the p1000 the smallest unit is 1 ul so you cannot set it to 871.3.
answered 6 hours ago
CellCell
362116
edited 2 hours ago
answered 6 hours ago
CellCell
362116
answered 6 hours ago
CellCell
362116
answered 6 hours ago
CellCell
362116
362116
$begingroup$
Some brands of pipeters "click" at each allowed increment, so you won't be able to go between them.
$endgroup$
– swbarnes2
4 hours ago
$begingroup$
Interesting. Thanks for sharing I didn't know that.
$endgroup$
– Cell
2 hours ago
add a comment |
$begingroup$
Some brands of pipeters "click" at each allowed increment, so you won't be able to go between them.
$endgroup$
– swbarnes2
4 hours ago
$begingroup$
Interesting. Thanks for sharing I didn't know that.
$endgroup$
– Cell
2 hours ago
$begingroup$
Some brands of pipeters "click" at each allowed increment, so you won't be able to go between them.
$endgroup$
– swbarnes2
4 hours ago
$begingroup$
Some brands of pipeters "click" at each allowed increment, so you won't be able to go between them.
$endgroup$
– swbarnes2
4 hours ago
$begingroup$
Interesting. Thanks for sharing I didn't know that.
$endgroup$
– Cell
2 hours ago
$begingroup$
Interesting. Thanks for sharing I didn't know that.
$endgroup$
– Cell
2 hours ago
add a comment |
Sara is a new contributor. Be nice, and check out our Code of Conduct.
Sara is a new contributor. Be nice, and check out our Code of Conduct.
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